G. Krens, S. Terjung, R. Nitschke ┊ Session 1. Cyber security, facility equipment maintenance and toolsG. Krens1,R. Nitschke,S. Terjung (1Imaging & Optics Facility (IOF), Institute of Science and Technology Austria (ISTA))
10:30- 11:00Coffee & tea break, sponsored by 3i - Intelligent Imaging InnovationsExhibition area
11:00- 12:15CF Day, Session 2. Staff development + trainingPlenary room
11:00 - 12:15
Oral
N. Halidi, M. Carvalho, T. Zimmermann ┊ Session 2: Staff development & trainingN. Halidi1,M.T. Carvalho,T. Zimmermann,M. Mertz (1Advanced Light Microscopy Unit, Centre for Genomic Regulation, Barcelona, Spain)
12:15- 13:30Lunch, sponsored by Chroma TechnologyExhibition area
13:30- 14:45CF Day, Session 3. Data Management: User experience & IT infrastructurePlenary room
13:30 - 14:45
Oral
C. Strambio De Castillia, M. Mertz, N. Halidi ┊ Session 3: Data Management: User experience & Research IT infrastructureN. Halidi,M. Mertz2,C. Caterina Strambio-De-Castillia (2BioImaging Facility, Netherlands Cancer Institute, Amsterdam, Netherlands)
14:45- 15:15Coffee & tea break, sponsored by ASI/Applied Scientific Instrumentation Inc.Exhibition area
15:15- 16:15CF Day, Session 4. (Global) Community Initiatives (All)Plenary room
Spatio-Temporal Interrogation of Signaling Networks Using Biosensor Imaging and OptogeneticsO.P. Pertz1 (1Institute of Cell Biology, University of Bern, Switzerland)
09:30 - 09:45
Oral
Selective degradation of ARF monomers controls auxin response inMarchantiaJ.W. Borst1,S. Das,M. Roij, de,D. Weijers (11Laboratory of Biochemistry, Wageningen University and Research, the Netherlands.)
09:45 - 10:00
Oral
Mobility of the giant protein nebulin in skeletal muscle sarcomeres using PALM and FRAPS.J.P. Bogaards1,M. Yuen,Y.L. Onderwater,C. Flores Clara,R.A. Galli,R.J. van der Pijl,S. shen,M. Vizoso,L. Nahidiazar,K. Jalink,J. van Rheenen,H.L. Granzier,C.A.C. Ottenheijm (1Amsterdam UMC location Vrije Universiteit Amsterdam, Department of Physiology, Amsterdam, The Netherlands)
10:00 - 10:15
Oral
RNA export through the nuclear pore complex is directionalY. Shav-Tal1 (1The Faculty of Life Sciences and the Institute for Nanotechnology and Advanced Materials (BINA), Bar-Ilan University, Ramat Gan, Israel)
10:15 - 10:30
Oral
A flat-field TIRF-SIM for combining live cell super-resolution with quantitative single molecule analysis.H. Winkelmann,J. Eising,J. Piehler,R. Kurre1 (1Department of Biology/Chemistry and Center for Cellular Nanoanalytics, Osnabrück University, Barbarastraße 11, D-49076 Osnabrück, Germany)
10:30- 10:45General discussionPlenary room
10:45- 11:15Coffee & exhibitionExhibition area
11:15- 12:15Workshop 1Different rooms
Workshop - Room C21
3i - Intelligent Imaging Innovations ┊ Living Cell Microscopy With Lattice LightSheetA. Barazia1 (13i - Intelligent Imaging Innovations)
Workshop - Room E7
Abbelight ┊ Simultaneous multi-color, quantitative and auto-calibrated TIRF imaging with the new Abbelight TIRF solutionM. Fournier1 (1Abbelight)
ACQUIFER Imaging GmbH ┊ The ACQUIFER IM – Workflows for Automated Imaging, Photomanipulation and High Content Screening J. Gehrig1,L. Thomas (1ACQUIFER Imaging GmbH)
Workshop - Room B13
Andor Technology ┊ Use Imaris AI-Powered Filament Tracing to Understand and Compare your Filamentous Structures A Paszulewicz1,M Price (1Andor, An Oxford Instruments Company)
Workshop - Room B17
ARGOLIGHT ┊ All you’ve ever wanted to know about spatial resolution and how to measure itA. Royon1,A. Egron,R. Ginet (1Argolight)
Workshop - Room B11
ASI ┊ Commercially-available single-objective light sheet microscopeJ.S. Daniels1 (1ASI/Applied Scientific Instrumentation, Eugene, OR, USA)
Workshop - Room C32
Bruker ┊ Fast whole organ imaging with light sheet microscopyJ. Mayer,B. Eismann,F. Weltzien1 (1Luxendo GmbH, Bruker Fluorescence Microscopy)
Workshop - Room C30
Carl Zeiss Microscopy ┊ Tracking the Ultrastructure of Life: Serial block-face imaging to investigate cells and tissue in 3DA. Elli1,N. Braun (1ZEISS Microscopy)
Workshop - Room C27
Confocal.nl ┊ Application of NL5+ by Confocal.nl in fixed or live cell confocal imagingA Feher,D Popov-Čeleketić1 (1Confocal.nl BV)
Workshop - Room B19
EVIDENT Europe ┊ Collaboration for Innovation: Introducing the VT-iSIM System for Super-Resolution ImagingS. Coleman1 (1VisiTech International Ltd. & EVIDENT Europe)
Workshop - Room E3
German BioImaging ┊ Illumination Stability and Linearity (QUAREP-LiMi WG 1) L. Gelman1,K. van der Oord,A. Fallisch (1Friedrich Miescher Institute for Biomedical Research, Facility for Advanced Imaging and Microscopy, Basel, CH)
Workshop - Room C23
INSCOPER ┊ Advanced automation software features for fluorescence imagingO.B. Bouchareb1 (1INSCOPER - Cesson-Sévigné, France)
Workshop - Room E5
Interherence ┊ QUSCITE: The stagetop TIRF module with square millimetres field-of-view and VAHEAT: precise temperature control for high-resolution microscopyA. Singh1,N. Fitzgerald,P. Türschmann (1Interherence GmbH, Erlangen, Germany)
Workshop - Room C25
Lambert Instruments ┊ LIFA, a versatile camera-based Frequency domain FLIM systemJ.S.A. Herz1 (1Lambert Instruments BV, Groningen, The Netherlands)
Multiple Routes to 3D Imaging – Combining Information from Different Microscopy ModalitiesK.S. Grussmayer1,2,3,A. Descloux,V. Navikas,R. Wombacher,K. Yserentant,S.M. Leitao,K. Ibrahim,N. Riguet,L. Feletti,D.P. Herten,G. Fantner,H. Lashuel,A. Randenovic (1Department of Bionanoscience and Kavli Institute of Nanoscience Delft, TU Delft, Netherlands; 2Laboratory of Nanoscale Biology, EPFL, Switzerland; 3Laboratory of Biomedical Optics, EPFL, Switzerland)
14:00 - 14:15
Oral
Medium Content FAST 3D RIM Superresolution Microscopy for live Biological Imaging : from cell culture to tissues.S. Labouesse,G. Giroussens,C. Estibal,K. affannoukoue,M. Allain,G. Legube,R. Poincloux,J. Idier,A. Sentenac,T. Mangeat1 (1LITC Core Facility, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, 31062 Toulouse, France)
14:15 - 14:30
Oral
Fast three-dimensional random access scanning using a MEMS-based spatial light modulatorC. Berlage1,2,U.L. Böhm,B. Judkewitz (1Charité - Universitätsmedizin Berlin, 10117 Berlin, Germany; 2Humboldt Universität zu Berlin, Institute for Biology, 10099 Berlin, Germany)
ACQUIFER Imaging ┊ The ACQUIFER HIVE - Solution for Large Image DataO. Selchow1 (1ACQUIFER Imaging GmbH)
Workshop - Room B13
Andor Technology ┊ Dragonfly 600: Imaging from protein complexes to model organismsC. Florindo1,B. Combettes (1Andor, An Oxford Instruments Company)
Workshop - Room B11
Becker & Hickl ┊ There and back again: the (surprisingly quick) path to quantitative fluorescence lifetime imaging resultsJ.J.F. Heitz1,M. Alhibah,H. Studier (1Becker&Hickl GmbH, Berlin, Germany)
Workshop - Room C32
Bruker ┊ How to get started with 3D-DNA-PAINT Superresolution MicroscopyC. Schneider1,F. Selbach,S. Strauss (1Bruker Fluorescence Microscopy)
Workshop - Room C30
Carl Zeiss Microscopy ┊ AI-assisted Image Analysis and Scalable Data Processing for Life Sciences: Boost your research with the ZEISS arivis scientific software platformM. McQuilken1 (1ZEISS Microscopy)
Workshop - Room C27
Confocal.nl ┊ Application of NL5+ by Confocal.nl in fixed or live cell confocal imagingA Feher,D Popov-Čeleketić1 (1Confocal.nl BV)
Workshop - Room B19
EVIDENT Europe ┊ Interactive 3D imaging assistant for multi-scale light sheet microscopyI. Rakotoson1 (1PhaseView & EVIDENT Europe)
Workshop - Room E3
German BioImaging ┊ PSF acquisition and analysis (QUAREP-LiMi WG 5) + FIELD HOMOGENEITY (QUAREP-LiMi WG 3) L. Plantard1,N. Halidi,L. Gelman,S. Ritz,M. Spitaler,I. Alexopoulos (1Friedrich Miescher Institute for Biomedical Research, Facility for Advanced Imaging and Microscopy, Basel, CH)
Workshop - Room C23
INSCOPER ┊ Multi-dimensional fluorescence imaging with the Zaber automated microscopeO.B. Bouchareb1 (1INSCOPER - Cesson-Sévigné, France)
Workshop - Room E5
Interherence ┊ QUSCITE: The stagetop TIRF module with square millimetres field-of-view and VAHEAT: precise temperature control for high-resolution microscopyA. Singh1,N. Fitzgerald,P. Türschmann (1Interherence GmbH, Erlangen, Germany)
Workshop - Room C25
Lambert Instruments ┊ LIFA, a versatile camera-based Frequency domain FLIM systemJ.S.A. Herz1 (1Lambert Instruments BV, Groningen, The Netherlands)
ACQUIFER Imaging ┊ Automated imaging with the ACQUIFER IM: versatile scripting opportunitiesL. Thomas1 (1ACQUIFER Imaging GmbH)
Workshop - Room B13
Andor Technology ┊ Diving into Structure and Dynamics: Learn how to use Imaris’ Spatial Statistics in Fixed and Live SamplesA. Paszulewicz1,M. Price (1Andor, An Oxford Instruments Company)
Workshop - Room B17
Argolight ┊ One size-only QC process? The good questions to define the QC process of fluorescence imaging systemsA. Egron1,R. Ginet,A. Royon (1Argolight)
Workshop - Room C32
Bruker ┊ Bring you up to data for your sample’s sake – TruLive3D ImagerM. Löschinger1 (1Luxendo GmbH, Bruker Fluorescence Microscopy)
Workshop - Room C30
Carl Zeiss Microscopy ┊ Driving new discoveries with Lattice Lightsheet 7L.C. Kapitein1,E Katrukha,K. Elgass (1Utrecht University, 3584 Utrecht, The Netherlands)
Workshop - Room B11
Chip NanoImaging ┊ Microscopy based on photonic chips giving ultra wide field of view in super resolution and diffraction limited mode.J.K. Hagene1,Ø.I. Helle (1Chip NanoImaging AS, Tromsø, Norway)
Workshop - Room C27
Confocal.nl ┊ Application of NL5+ by Confocal.nl in fixed or live cell confocal imagingA Feher,D Popov-Čeleketić1 (1Confocal.nl BV)
Workshop - Room B19
EVIDENT Europe ┊ VS200 SILA: optical sectioning for whole slide imagingM. Prusicki1 (1EVIDENT Technology Center Europe GmbH)
Workshop - Room E3
German BioImaging ┊ Detection System Performance - Camera topics (QUAREP-LiMi WG 2)S. Beer1,G. Holst,G.J. Bakker,D. McFadden (1Hamamatsu Photonics Deutschland GmbH, Herrsching, DE)
Workshop - Room C23
INSCOPER ┊ Multi-colour confocal microscopy with the new Hamamatsu MAICOG.B. Bertholin1 (1Univ Rennes, CNRS, IGDR (Institute of Genetics and Development of Rennes), UMR 6290, Rennes, F- 35000 France)
Workshop - Room E5
Interherence ┊ QUSCITE: The stagetop TIRF module with square millimetres field-of-view and VAHEAT: precise temperature control for high-resolution microscopyA. Singh1,N. Fitzgerald,P. Türschmann (1Interherence GmbH, Erlangen, Germany)
Workshop - Room C25
Lambert Instruments ┊ LIFA, a versatile camera-based Frequency domain FLIM systemJ.S.A. Herz1 (1Lambert Instruments BV, Groningen, The Netherlands)
Workshop - Room B9
Leica Microsystems ┊ Access the future of AI microscopyP. Mascalchi1,Q. Tran,S. McElroy,WY. Choi,L.A.G. Lucas (1Leica Microsystems, France)
Workshop - Room B12
Nikon Europe ┊ Reveal new confocal imaging potential with NSPARC, the new AX confocal detectorS. Delattre1 (1Nikon Europe B.V.)
Workshop - Room C31
PicoQuant ┊ How Luminosa can increase the reproducibility of your confocal imagingE. Sisamakis1,M. Bayer,F. Jolmes,M. Patting,M. Koenig,M. Sackrow,U. Ortmann,F. Koberling,R. Erdmann (1PicoQuant GmbH, Rudower Chaussee 29, 12489 Berlin, Germany, luminosa@picoquant.com)
18:00- 19:00Posters & drinksPPoster area
P / 1
Poster
Napari-live-recording: embedding camera acquisition in napariJ. Abramo1,P. Pritzke,F. Wanitschke,F. Reina,C. Eggeling (1Leibniz-Institute for Photonic Technology, Jena, Germany)
P / 2
Poster
Visualisation of nano-structures using Expansion MicroscopyA. Agostinho1,S. Edwards,H. Blom,H. Brismar (1Science for Life Laboratory, Department of Applied Physics, Royal Institute of Technology, Solna, Sweden)
P / 3
Poster
The effects of vitamin D treatment on liver degeneration and pyroptosis in rats fed with high-fat and high-fructose diet.N. Bayramova1,B Zorkaya,S. Rzayeva,T. Ozbilenler,A.S. Akdemir,E. Bayrak Kömürcü,M. Ozturk,F. Kaya Dagistanli (1Department of Medical Biology, Cerrahpasa Faculty of Medicine, Istanbul University-Cerrahpasa, Istanbul, Turkey)
P / 4
Poster
Clearing the way: Non-toxic clearing and labeling with fluorescent REAfinityTM antibodies for enhanced 3D visualization of tissues and organsK. Bigott1,J. Werheid,K. Gerhold,F. Küster,D. Barleben,D. Bessa-Neto,D.A. Yushchenko,L. Haalck,G. Ebel,C. Dose,A. Bosio,M. Jungblut,L. Nolte,D. Eckardt (1Miltenyi Biotec GmbH, Bergisch Gladbach, Germany)
P / 5
Poster
How Euro-BioImaging can support your research with access to the best imaging toolsJ. Bischof1,M. Childress-Poli,A. Mathur,C. Pfander,A. Keppler (1Euro-BioImaging ERIC)
P / 6
Poster
3 scopes with 1 scope: long-term live imaging of viruses in plantsG. Bradamante1,A. Moreno Cencerrado,P. Pasierbek,R. Hauschild,K. Riha,O. Mittelsten Scheid,M. Incarbone (1Gregor Mendel Institute (GMI))
P / 7
Poster
Towards Community Standards in Adaptive Feedback MicroscopyB. Balazs,J. Bischof,R. Camacho3,M. Gunkel,A. Halavatyi,C. Pfander,H. Schaden,E. Sisamakis,M. Tewinkel,L. Thomas (3Center for Cellular Imaging, Core Facilities, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden)
Development of a custom laser ablation microscopeY. Cesbron1,N. Darwish,R. Hauschild,S.F.G. Krens (1Imaging and Optics Facility, Institute of Science and Technology Austria, Klosterneuburg, Austria)
P / 10
Poster
Center for Advanced Bioimaging (CAB)N.M. Christensen1,S.J. Kjeldgaard-Nintemann (1Center for Advanced Bioimaging, Copenhagen University, DK)
P / 11
Poster
Illuminating the truth behind fluorescent labels: The unfriendly side of “live-cell friendly” probesP.D. Djali1,A.D. Dickinson (1Phasefocus, The Electric Works Concourse Way, Sheaf St, Sheffield City Centre, Sheffield S1 2BJ)
P / 12
Poster
Superconducting nanowire single-photon detectors (SNSPDs) for microscopyM. Caldarola,A. Fakhree1,A. Guardiani,H. Kooiman,A. Fognini (1Single Quantum B.V., Delft, The Netherlands)
P / 13
Poster
A three-photon excitation fluorescence microscope for in vivo deep tissue imaging.H. Fried1,S.K. Kaushalya,R. Kasper,K. Keppler,F. Fuhrmann,F. Musacchio,N. Reichenbach,G. Petzold,M. Fuhrmann,E. Fava (1Core Research Facilities and Services, Light Microscope Facility, German Center for Neurodegenerative Diseases (DZNE))
P / 14
Poster
mScarlet3: a brilliant and fast maturing red fluorescent protein for cellular imagingT.W.J. Gadella1,L. van Weeren,J. Stouthamer,M.A. Hink,A.M.G. Wolters,B.N.G. Giepmans,S. Aumonier,J. Dupuy,A. Royant (1Section of Molecular Cytology and van Leeuwenhoek Centre for Advanced Microscopy , Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, the Netherland)
P / 15
Poster
Challenges of imaging human brain sections with Light Sheet MicroscopyI. Gantar1,T. Jordà-Siquier,J. Scholler,L. Batti,C. Lamy,S. Pagès (1The Wyss Center for Bio and Neuroengineering, Geneva, Switzerland)
P / 16
Poster
Enabling Spectrally Resolved Single-Molecule Localization Microscopy at High Emitter Densities in a 4f GeometryJ. Hohlbein1,2 (1Laboratory of Biophysics, Wageningen University and Research, The Netherlands; 2Microspectroscopy Research Facility, Wageningen University and Research, The Netherlands)
Phenospot (Photonic hyperspectral no-label spotter); label-free, real-time in-situ monitoring of live-cell protein specific secretion.A.G. Kay1,I. Barth,A.H. Cull,L.M. Miller,C. Reardon,S. Johnson,P.J. O'Toole,T.F. Krauss (1Department of Biology, University of York, York, UK)
P / 20
Poster
Plant imaging at the Center for Advanced Bioimaging (CAB)S.J. Kjeldgaard-Nintemann1,N.M. Christensen (1Center for Advanced Bioimaging Denmark, Faculty of Science, University of Copenhagen, Denmark)
P / 21
Poster
Integration Workflow of the Deformable Phase Plate for Enhancing Light Microscopy by Adaptive OpticsS. Klykov1,P. Rajaeipour (1Phaseform GmbH, Georges-Köhler-Allee 102, 79110 Freiburg im Breisgau, Germany)
P / 22
Poster
Light sheet microscopy of cleared Drosophila larvaeO.K. Kobler1,T.S. Stöter,B.G. Gerber,U.T. Thomas (1Leibniz Institute for Neurobiology, Department Combinatorial NeuroImaging Core Facility (CNI), Magdeburg)
P / 23
Poster
Simulator of a Confocal in FIJI (SCiF), a teaching tool for laser scanning confocal microscopyG.J. Kremers1,J.A. Slotman,W.A. van Cappellen,A.B. Houtsmuller (1Erasmus Optical Imaging, Erasmus MC, Rotterdam, the Netherlands)
P / 24
Poster
Super-resolution capacity of variance-based stochastic fluorescence microscopy.S. Labouesse1,J. Idier,M. Allain,G. Giroussens,T. Mangeat,A. Sentenac (1LITC Core Facility, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, 31062 Toulouse, France)
P / 25
Poster
Blind Point Spread Function and Illumination in Image Scanning MicroscopyS. Labouesse1,T. Mangeat,E. Premillieu,R. Piestun2 (1LITC Core Facility, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, 31062 Toulouse, France)
P / 26
Poster
Super-Resolved Total Internal Reflection Fluorescence Microscopy using Random Illuminations, TIRFRIM K. Affannoukoué,S. Labouesse,G. Maire,L. Gallais,J. Savatier,M. Allain,L. Le Goff,M.D. Rasedujjaman,R. Poincloux,J. Idier,C. Leterrier,T. Mangeat2,A. Sentenac (2LITC Core Facility, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, 31062 Toulouse, France)
P / 27
Poster
Assessing co-localisation of nuclear proteins with super resolution microscopy: a comparison between Expansion Microscopy and dSTORMM. Marenda1,M. Russo,D. Polizzese,C. Borriero,G. Natoli,S. Rodighiero (1IEO, European Institute of Oncology IRCCS, Department of Experimental Oncology, Via Adamello 16, 20139 Milan, Italy)
P / 28
Poster
Building a FAIR image data ecosystem for microscopy communitiesI. Kemmer,A. Keppler,B. Serrano-Solano,A. Rybina,B. Özdemir,J. Bischof,A. El Ghadraoui,J.E. Eriksson,A. Mathur1 (1Euro-BioImaging ERIC Bio-Hub, European Molecular Biology Laboratory (EMBL) Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany)
P / 29
Poster
Accelerating Scientific Progress through Open Science: Sharing Documentation for Continuous Development of Open-Source Microscopy in Live-Cell and Single-Molecule Fluorescence ImagingC.N. Niederauer1,M.S. Seynen,J.Z. Zomerdijk,M.K. Kamp,K.A.G. Ganzinger (1AMOLF, Amsterdam)
P / 30
Poster
A comparison of super-resolution microscopy approaches to undertake deep learning-based 3D shape analysis of Orientia tsutsugamushi bacteria inside mammalian cellsA. North1,C. Pyrgaki,V. Sharma,J. Lim,S. Atwal,K. Saharat,S. Lee,G. Wright,J. Salje (1Bio-Imaging Resource Center, The Rockefeller University, New York, NY USA)
P / 31
Poster
The Vanishing Act of RACK1: Insights from Quantitative Phase ImagingT. Groušl,V. Chvalová,A. Křížová,Z. Nováková2 (2Telight, R&D Applications, Brno, Czech Republic)
P / 32
Poster
CellPhe - A Cell Phenotyping App and pattern recognition toolkitL. Wiggins,W.J. Brackenbury,J. Wilson,P.J. O'Toole1 (1York Biomedical Research Institute, University of York, York, UK)
P / 33
Poster
BioImaging North America Metrology Suitcase Pilot ProgramV.L. Orr1,N. Bialy,C. Strambio De Castillia,A.J. North,C.M. Brown,K.W. Eliceiri (1Morgridge Institute for Research, Madison, Wisconsin, USA)
P / 34
Poster
A comprehensive routine quality check protocol for facility microscopes using Argolight Calibration Slides.M. Oster1,M. Spitaler (1Max Planck Institute of Biochemistry - Imaging Facility, Martinsried (Munich), Germany)
P / 35
Poster
Applications of conical diffraction to super-resolution fluorescence microscopyC. Peron-Cane1,2,A.B. Schroeder,T. Sperber,G. Sirat (1Telight, Libušina třída 21, Brno 623 00, Czech Republic; 2BioAxial SAS, 174 Quai de Jemmapes, 75010 Paris, France)
P / 36
Poster
Euro-BioImaging – access to the imaging community (not only) for industryC Pfander1 (1Euro-BioImaging)
P / 37
Poster
Linearmotor as a key-component in the microscopyH. Rausch1 (1ITK Dr. Kassen GmbH)
P / 38
Poster
Introducing the German BioImaging Society for Microscopy and Image Analysis (GerBI-GMB) Team Intravital Microscopy K.R. Roth1,I.A. Alexopoulos,G.B. Bakker,H.F. Fried,A.G. Gheisari,H.I. Ishikawa-Ankerhold,M.M. Mitkovksi,C.P. Peukert,M.P. Pieper,B.S. Stolp,S.T. Tulok,M.G. Gerlach (1Center for Tumor and Immune Biology, Philipps University Marburg, Marburg, Germany)
P / 39
Poster
Optimization of Advanced Live-Cell Imaging Through Red/Near-Infrared Dye Labeling and Fluorescence Lifetime-Based StrategiesM.B. Bénard,D.S. Schapman1,C.C. Chamot,F.D. Dubois,G.L. levallet,H.K. Komuro,L.G. Galas (1PRIMACEN, INSERM, UNIROUEN, Normandie University, 76000 Rouen, France)
P / 40
Poster
Decontamination of BSL3 confocal microscope systemsO.M. Schwartz1,J. Kabat (1Biological Imaging Facility, RTB, National Institute of Allergy and Infectious Diseases , NIH, Bethesda, Maryland, USA)
P / 41
Poster
Will label-free hyperspectral imaging become a routine technique in living cells? A. Hochstetter,K.A. Sjollema1,2,B.N.G. Giepmans (1UMC Groningen Microscopy & Imaging Center (UMIC), UMC Groninghen, The Netherlands; 2Dept. Biomedical Sciences of Cells & Systems, UMC Groningen, The Netherlands)
P / 42
Poster
Quantum optics meets microscopy – An ultra-sensitive resonator microscope for nano- and life sciencesF. Steiner1,R. Fernandes,J. Noe,T. Hümmer (1Ludwig-Maximilians-University Munich, Department of Physics, Munich, Germany)
P / 43
Poster
Deep-tissue live-imaging in plant organs with multi-photon microscopyA.C.J. Timmers1,C. Gough,S. Bensmihen (1Central Microscopy, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Cologne, Germany)
P / 44
Poster
Towards integral mapping of glioblastoma cell invasion in cerebral organoidsT. Van De Looverbosch1,S. De Beuckeleer,S. Peeters,J. Van Den Daele,J. Sijbers,W. De Vos (1Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, Antwerp, Belgium)
P / 45
Poster
DEEPBleND - A CNN for subtype classification of nuclear dysmorphy M. Verschuuren1,2,3,T. Van De Looverbosch,S. Peeters,F. Molenberghs,W.H. De Vos (1Laboratory of Cell Biology & Histology, University of Antwerp, Department of Veterinary Sciences, Antwerp, Belgium; 2Antwerp Centre for Advanced Microscopy, University of Antwerp, Antwerp, Belgium; 3µNEURO Research Excellence Centre, University of Antwerp, Antwerp, Belgium)
P / 46
Poster
The EMBL Imaging Centre, open access to cutting-edge electron and light microscopyD.W.M Walsh1,V. Pierini (1EMBL Imaging Centre, Heidelberg, Germany)
P / 47
Poster
Versatile tool collection for license usage analysis and management, and IT maintenance in core facilities.T.W. Wernet1,2,A.F. Fallisch,A.N. Naumann,I.B. Bierschenk,R.N. Nitschke (1Albert-Ludwigs-Universität Freiburg; 2I3D:bio – Information Infrastructure for BioImage Data)
P / 48
Poster
Imaging metabolic dynamics by label-free wide-field FLIM of NADH and FAD autofluorescenceA. Weber,E. Altun,A. Wetzel,A. Bikbaev,A. Luarte,W. Zuschratter1 (1Leibniz Inst. for Neurobiology., Magdeburg, Germany)
P / 49
Poster
Objective quality assessment workflow using ArgoLight slide and OMEROR. Dornier1,R. Guiet,T. Laroche,J. Artacho,A. Seitz (1BIOP, EPFL)
19:30- 21:30Dinner (sponsored by Zeiss)
22:00-01:00 Drinks (own account) and karaokeSportscafe Cheers
Thursday, 8th of June
08:00-17:30 RegistrationRegistrationExhibition area
A FAIR Outlook for NL-BioimagingK.J. Wolstencroft1 (1Leiden Institute of Advanced Computer Science, Leiden University)
09:30 - 10:00
Invited
OME-Zarr: a cloud-optimized bioimaging file format with international community supportJ. Moore1,2 (1German BioImaging-Gesellschaft für Mikroskopie und Bildanalyse e.V.; 2Open Microscopy Environment (OME))
10:00 - 10:15
Oral
Unlocking FAIR image data - the BioImage Archive, powered by Recommended Metadata for Biological ImagesM. Hartley1 (1European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI))
10:15 - 10:30
Oral
A service architecture for bio-image data management at France BioImagingG. Gay1,2,JF. Guillaume,G. Maucort,M. Mongy,E. Faure,P. Paul-Gilloteaux (1BioCampus, UAR 3426 CNRS - US 09 INSERM - UM, Montpellier, France; 2LIRMM, UMR 5506 CNRS, INRIA, UM, Montpellier, France)
10:30- 10:45General discussionPlenary room
10:45- 11:15Coffee & exhibitionExhibition area
11:15- 12:15Workshop 4Different rooms
Workshop - Room C21
3i - Intelligent Imaging Innovations ┊ Living Cell Microscopy With Lattice LightSheetA. Barazia1 (13i - Intelligent Imaging Innovations)
Workshop - Room E7
Abbelight ┊ Simultaneous multi-color, quantitative and auto-calibrated TIRF imaging with the new Abbelight TIRF solutionM. Fournier1 (1Abbelight)
ACQUIFER Imaging ┊ Practical Workshop on image data handling - The ACQUIFER HIVE O. Selchow1 (1ACQUIFER Imaging GmbH)
Workshop - Room B13
Andor Technology ┊ Dragonfly 600: Imaging from protein complexes to model organismsC. Florindo1,B. Combettes (1Andor, An Oxford Instruments Company)
Workshop - Room B17
Argolight ┊ One size-only QC process? The good questions to define the QC process of fluorescence imaging systemsA. Egron1,R. Ginet,A. Royon (1Argolight)
Workshop - Room C32
Bruker ┊ How to get started with 3D-DNA-PAINT Superresolution MicroscopyC. Schneider1,F. Selbach,S. Strauss (1Bruker Fluorescence Microscopy)
Workshop - Room C30
Carl Zeiss Microscopy ┊ Driving new discoveries with Lattice Lightsheet 7L.C. Kapitein1,E Katrukha,K. Elgass (1Utrecht University, 3584 Utrecht, The Netherlands)
Workshop - Room C27
Confocal.nl ┊ Application of NL5+ by Confocal.nl in fixed or live cell confocal imagingA Feher,D Popov-Čeleketić1 (1Confocal.nl BV)
Workshop - Room B19
EVIDENT Europe ┊ Collaboration for Innovation: Introducing the VT-iSIM System for Super-Resolution ImagingS. Coleman1 (1VisiTech International Ltd. & EVIDENT Europe)
Workshop - Room B11
Excelitas PCO ┊ SWIR Cameras & Optimum Illumination for Macroscopy and MicroscopyG. Holst1,K. Aswani (1Excelitas)
Workshop - Room E3
German BioImaging ┊ Data Management for Bioimaging Data and Metadata Annotation ToolsS. Kunis,J. Lacoste,C. Strambio-De-Castillia3,S. Weidtkamp-Peters (3Program in Molecular Medicine, University of Massachusetts Medical School, MA, US)
Workshop - Room C23
INSCOPER ┊ Multi-dimensional fluorescence imaging with the Zaber automated microscopeO.B. Bouchareb1 (1INSCOPER - Cesson-Sévigné, France)
Workshop - Room E5
Interherence ┊ QUSCITE: The stagetop TIRF module with square millimetres field-of-view and VAHEAT: precise temperature control for high-resolution microscopyA. Singh1,N. Fitzgerald,P. Türschmann (1Interherence GmbH, Erlangen, Germany)
Workshop - Room C25
Lambert Instruments ┊ LIFA, a versatile camera-based Frequency domain FLIM systemJ.S.A. Herz1 (1Lambert Instruments BV, Groningen, The Netherlands)
Workshop - Room B9
Leica Microsystems ┊ All Fluorophores Welcomed: How to extend confocal microscopy with multicolor unmixing tools for advanced multiplexing and spatial omicsJ. Roberti,S. Hoffmann,I. Steinmetz,J. Reddington,L.A.J. Alvarez1 (1Leica Microsystems CMS, Am Friedensplatz 3, 68165, Mannheim, Germany.)
Workshop - Room B14
Miltenyi Biotec ┊ "Dive deep into your sample – the Miltenyi 3D imaging workflow"A Khalili1,G Arulmozhivarman (1Miltenyi Biotec B.V. & Co. KG)
Workshop - Room B12
A new spin on TIRF microscopy - Nikon integrates the ILas-2E. Miron1 (1Nikon Europe B.V.)
Workshop - Room C31
PicoQuant ┊ Using FLIM with confidence and minimal user interactionF. Jolmes1,E. Sisamakis,M. Bayer,M. Patting,M. Wahl,M. Sackrow,U. Ortmann,F. Barachati,F. Koberling,R. Erdmann (1PicoQuant GmbH, Rudower Chaussee 29, 12489 Berlin, Germany, luminosa@picoquant.com)
12:15- 13:30Lunch & exhibition, sponsored by Chroma TechnologyExhibition area
13:15- 13:30Euro-BioImaging meetingPlenary room
13:30- 15:00Intravital & light sheet microscopy (Invited: Jan Huisken)Plenary room
13:30 - 14:00
Invited
Flamingo: Modular and portable light sheet microscopy framework for novel biological experiments inside and outside the optics labM. Weber,J. Li,K. Weiss,R.M. Power,T. Bakken,P. Maier,G.F. Müller,M. Aakhte,M. Handberg-Thorsager,T. Göpel,J. Huisken1,2 (1Multiscale Biology, Dept. of Biology and Psychology, Georg-August-University Göttingen, Germany; 2Morgridge Institute for Research, Madison, WI, USA)
14:00 - 14:15
Oral
Imaging long-range neuronal connections in the mouse brain by light sheet fluorescence expansion microscopyJ.E. Rodriguez-Gatica1,M.K Schwarz,U. Kubitscheck (1Clausius Institute of Physical and Theoretical Chemistry, University of Bonn, Bonn, Germany)
14:15 - 14:30
Oral
soSPIM image enhancement methods for 3D cell cultures imagingT. Delaire1,I. Idrissi,G. Grenci,V. Viasnoff,R. Heintzmann,R. Galland,J.B. Sibarita (1Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, 33000 Bordeaux, France)
14:30 - 14:45
Oral
Through the Looking Glass: Imaging Animal Models of Human DiseasesO Kamenyeva1,J Kabat,O.M. Schwartz (1NIH/NIAID, Research Technology Branch, Biological Imaging Section)
14:45 - 15:00
Oral
Metabolic- and Malignancy-mediated Akt signaling revealed by Akt-FRET biosensor mouseJ.R.W. Conway,S.C. Warren,Y.K. Lee,A.T. McCulloch,A. Magenau,V. Lee,X.L. Metcalf,J. Stoehr,K. Haigh,L. Abdulkhalek,C.S. Guaman,D.A. Reed,K.J. Murphy,B.A. Pereira,P. Melenec,C.R. Chambers,S.L. Latham,H. Lenthall,E.K. Deenick,Y. Ma,E. Lim,A.M. Joshua,S. Walters,S.T. Grey,Y.C. Shi,L. Zhang,H. Herzog,D.R. Croucher,A. Philp,C.L.G.J. Scheele,D. Herrmann,O.J. Sansom,J.P. Morton,A. Papa,J.J. Haigh,M. Nobis1,2,14,P. Timpson (1Garvan Institute of Medical Research & The Kinghorn Cancer Centre, Sydney, NSW 2010, Australia; 2St Vincent’s Clinical School, Faculty of Medicine, UNSW Sydney, Sydney, NSW 2010, Australia; 14Intravital Imaging Expertise Center, VIB Center for Cancer Biology, KU Leuven, 3000 Leuven, Belgium)
15:00- 15:15General discussionPlenary room
15:15- 15:45Coffee & exhibitionExhibition area
15:45- 16:45Workshop 5Different rooms
Workshop - Room C21
3i - Intelligent Imaging Innovations ┊ Living Cell Microscopy With Lattice LightSheetA. Barazia1 (13i - Intelligent Imaging Innovations)
Workshop - Room E7
Abbelight ┊ Reliable SMLM cell imaging demonstrated on Abbelight SAFe imaging platform. N. BOURG1 (1ISMO, Orsay)
ACQUIFER Imaging GmbH ┊ The ACQUIFER IM – Workflows for Automated Imaging, Photomanipulation and High Content Screening J. Gehrig1,L. Thomas (1ACQUIFER Imaging GmbH)
Workshop - Room B13
Andor Technology ┊ Unlock the Potential of your Microscopy Data using Machine Learning in ImarisA. Paszulewicz1,M. Price (1Andor, An Oxford Instruments Company)
Workshop - Room B17
ARGOLIGHT ┊ All you’ve ever wanted to know about spatial resolution and how to measure itA. Royon1,A. Egron,R. Ginet (1Argolight)
Workshop - Room C32
Bruker ┊ Fast whole organ imaging with light sheet microscopyJ. Mayer,B. Eismann,F. Weltzien1 (1Luxendo GmbH, Bruker Fluorescence Microscopy)
Workshop - Room C30
Carl Zeiss Microscopy ┊ Tracking the Ultrastructure of Life: Serial block-face imaging to investigate cells and tissue in 3DA. Elli1,N. Braun (1ZEISS Microscopy)
Workshop - Room C27
Confocal.nl ┊ Application of NL5+ by Confocal.nl in fixed or live cell confocal imagingA Feher,D Popov-Čeleketić1 (1Confocal.nl BV)
Workshop - Room B19
EVIDENT Europe ┊ Interactive 3D imaging assistant for multi-scale light sheet microscopyI. Rakotoson1 (1PhaseView & EVIDENT Europe)
Workshop - Room E3
German BioImaging ┊ Hands-on metadata annotation for bioimaging using a REMBI-based annotation template T. Boissonnet1,J. Dohle,C. Christian Schmidt,C. Strambio-De-Castillia,T. Wernet (1Center for Advanced Imaging, Heinrich-Heine- University Düsseldorf, DE)
Workshop - Room C23
INSCOPER ┊ Multi-colour confocal microscopy with the new Hamamatsu MAICOG.B. Bertholin1 (1Univ Rennes, CNRS, IGDR (Institute of Genetics and Development of Rennes), UMR 6290, Rennes, F- 35000 France)
Workshop - Room E5
Interherence ┊ QUSCITE: The stagetop TIRF module with square millimetres field-of-view and VAHEAT: precise temperature control for high-resolution microscopyA. Singh1,N. Fitzgerald,P. Türschmann (1Interherence GmbH, Erlangen, Germany)
Workshop - Room C25
Lambert Instruments ┊ LIFA, a versatile camera-based Frequency domain FLIM systemJ.S.A. Herz1 (1Lambert Instruments BV, Groningen, The Netherlands)
ACQUIFER Imaging ┊ The ACQUIFER HIVE - Solution for Large Image DataO. Selchow1 (1ACQUIFER Imaging GmbH)
Workshop - Room B13
Andor Technology ┊ Democratizing Confocal Microscopy: A Fast Benchtop Microscope to Boost Productivity. G. Wilde1,C. Florindo (1Andor, An Oxford Instruments Company)
Workshop - Room C32
Bruker ┊ Bring you up to data for your sample’s sake – TruLive3D ImagerM. Löschinger1 (1Luxendo GmbH, Bruker Fluorescence Microscopy)
Workshop - Room C30
Carl Zeiss Microscopy ┊ AI-assisted Image Analysis and Scalable Data Processing for Life Sciences: Boost your research with the ZEISS arivis scientific software platformM. McQuilken1 (1ZEISS Microscopy)
Workshop - Room C27
Confocal.nl ┊ Application of NL5+ by Confocal.nl in fixed or live cell confocal imagingA Feher,D Popov-Čeleketić1 (1Confocal.nl BV)
Workshop - Room B19
EVIDENT Europe ┊ VS200 SILA: optical sectioning for whole slide imagingM. Prusicki1 (1EVIDENT Technology Center Europe GmbH)
Workshop - Room E3
German BioImaging ┊ Community-developed checklists for publishing images and image analysis (QUAREP-LiMi WG 12)C. Schmied1,2,B. Cimini (1Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, DE; 2Fondazione Human Technopole (HT), Milano, IT)
Workshop - Room C23
INSCOPER ┊ Advanced automation software features for fluorescence imagingO.B. Bouchareb1 (1INSCOPER - Cesson-Sévigné, France)
Workshop - Room E5
Interherence ┊ QUSCITE: The stagetop TIRF module with square millimetres field-of-view and VAHEAT: precise temperature control for high-resolution microscopyA. Singh1,N. Fitzgerald,P. Türschmann (1Interherence GmbH, Erlangen, Germany)
Workshop - Room C25
Lambert Instruments ┊ LIFA, a versatile camera-based Frequency domain FLIM systemJ.S.A. Herz1 (1Lambert Instruments BV, Groningen, The Netherlands)
Workshop - Room B9
Leica Microsystems ┊ All Fluorophores Welcomed: How to extend confocal microscopy with multicolor unmixing tools for advanced multiplexing and spatial omicsJ. Roberti,S. Hoffmann,I. Steinmetz,J. Reddington,L.A.J. Alvarez1 (1Leica Microsystems CMS, Am Friedensplatz 3, 68165, Mannheim, Germany.)
Workshop - Room B12
Nikon Europe ┊ Adding SIM to a Spinning Disk has never been that easyC. Rosazza1 (1Nikon Europe B.V.)
Workshop - Room C31
PicoQuant ┊ How Luminosa can increase the reproducibility of your confocal imagingE. Sisamakis1,M. Bayer,F. Jolmes,M. Patting,M. Koenig,M. Sackrow,U. Ortmann,F. Koberling,R. Erdmann (1PicoQuant GmbH, Rudower Chaussee 29, 12489 Berlin, Germany, luminosa@picoquant.com)
Workshop - Room B11
Tokai Hit ┊ The system you need for Live Cell Imaging: Introduction of incubation systems & Fluidic options for Dynamic culturing.K.N. Nishioka1,T.T. Tsuchiya1 (1TOKAI HIT Co., Ltd., Shizuoka, JAPAN)
08:00-14:30 RegistrationRegistrationExhibition area
09:00- 10:30Multi-modal imaging (Invited: Martina Schifferer)Plenary room
09:00 - 09:30
Invited
Array tomography enables correlative volume electron microscopy and spatial transcriptomicsM. Schifferer1 (1German Center for Neurodegenerative Diseases)
09:30 - 09:45
Oral
Towards high accuracy µCT-LM-EM correlative workflow with fluorescent µCT contrastY. Belyaev,F. Meyenhofer,B. Egger,R. Hlushchuk3 (3Institute of Anatomy, University of Bern, Switzerland)
09:45 - 10:00
Oral
Correlative Light and Electron Microscopy of whole-central nervous system in Drosophila adultsM. Marcello1,A. Beckett,M. Held,T. Waring,L. Isherwood,G. Dykes,T. Zech,I. Prior,N. Sanchez-Soriano (1Liverpool Shared Research Facilities, University of Liverpool, UK)
10:00 - 10:15
Oral
Improving super resolution cryo-CLEML.M. Voortman1,M.G.F. Last,T.H. Sharp (1Dept. of Cell and Chemical Biology, Leiden University Medical Center, Leiden, the Netherlands)
10:15 - 10:30
Oral
Automated segmentation of cellular ultrastructure in large scale ColorEMB.H.P. Duinkerken1,A.M.J.A. Alsahaf,J.P. Hoogenboom,B.N.G. Giepmans (1University Medical Center Groningen, Dept. Biomedical Sciences of Cells and Systems, Groningen, the Netherlands)
10:30- 10:45General discussionPlenary room
10:45- 11:15Coffee & exhibitionExhibition area
11:15- 12:45High content imaging (Invited: Rafael Carazo Salas)Plenary room
11:15 - 11:45
Invited
Predicting ‘live’ cell fate dynamics in human stem cells by deep learning-enhanced morphological profilingR.E. Carazo Salas1 (1School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK)
11:45 - 12:00
Oral
Systems microscopy of neurodegeneration at multiple scalesM. Verschuuren1,2,3,S. De Beuckeleer,T. Van De Looverbosch,P. Verstraelen,S. Thys,I. Pintelon,J.P. Timmermans,W.H. De Vos (1Antwerp Centre for Advanced Microscopy, University of Antwerp, Antwerp, Belgium; 2Laboratory of Cell Biology & Histology, University of Antwerp, Department of Veterinary Sciences, Antwerp, Belgium; 3µNEURO Research Excellence Centre, University of Antwerp, Antwerp, Belgium)
12:00 - 12:15
Oral
Adapting high-content microscopy screening to study dynamic signaling mechanismsS. Mukherjee1,2,O. Kukk,J. Klarenbeek,B. van den Broek,K. Jalink (1Netherlands Cancer Institute, Amsterdam, The Netherlands; 2Swammerdam Institute of Life Sciences, University of Amsterdam, Amsterdam, The Netherlands)
12:15 - 12:30
Oral
Cell instance segmentation with deep learning using sparse annotationsK. Sugawara1,2,3,M. Averof (1RIKEN Center for Biosystems Dynamics Research, Kobe, Japan; 2Institut de Génomique Fonctionnelle de Lyon (IGFL), Lyon, France; 3Centre National de la Recherche Scientifique (CNRS), Paris, France)
12:30 - 12:45
Oral
Piximi: an in-browser "Images to Discovery" tool for easy deep learningB.A. Cimini1 (1Imaging Platform, Broad Institute)
If you agree to continue, you will be redirected to the payment platform Mollie, which will take care of the transation. After the payment is completed successfully, you will be redirected back to this page.